GUAVA® EASYCYTE SYSTEMS – Expanding the Potential of Flow Cytometry

by Jun 18, 2018Life Science

Unleash What’s Possible

Over fifteen years ago, Guava Technologies introduced the first compact benchtop flow cytometers. Today, the Guava® easyCyte line has been updated to offer up to 3 lasers and 14 parameters with greater sensitivity and optional high throughput capabilities. Powered by intuitive software, the Guava® easyCyte flow cytometers are some of the most dynamic and flexible benchtop systems available.

  • Up to 3 lasers and 14 parameters on a benchtop instrument
  • Microcapillary fluidics design eliminates sheath fluid and waste carboys
  • Accurate absolute cell counts via microcapillary and positive displacement syringe pump
  • Intuitive software includes comprehensive cell-health related assays
  • Detection of particles as small as 0.2 μm
  • High-throughput option for walk-away acquisition of up to 96 samples

How it Works

The Guava® easyCyte systems use patented, microcapillary, laser-based technology capable of detecting mammalian and microbial cells, particles, and beads. A sample of fluorescently labeled cells is aspirated into a uniquely proportioned microcapillary flow cell. Forward and side scatter characteristics are detected by photodiode, and fluorophores excited by the violet, blue, or red laser emit signals that are spectrally filtered to resolve up to 10 fluorophores simultaneously.
Sensitive and specific
Spherotech 8-color beads analyzed on the Guava® easyCyte 12HT system demonstrate the instrument’s proficiency for resolving adjacent fluorophores in multiple detection channels.

Immunological Phenotyping
10 μL adult human blood was stained for 20 minutes at room temperature with a cocktail containing anti-CD45 PerCP-Cy5.5, anti-CD3 Brilliant™ Violet 421, anti-CD4 PE-Cy7, anti-CD8 APC-Cy7, anti-CD16+CD56 PE, anti-CD19 Brilliant™ Violet 510, anti-CD45 RA APC, and anti-CD62L FITC. After incubation, cells were lysed and fixed with 180 μL Guava® lysing solution for 15 minutes at room temperature. Samples were then acquired on the Guava® easyCyte 12HT system. Lymphocytes identified as CD45+ were selected and subsequently gated into a SSC vs. CD3 plot. T cells (CD3+ and CD45+) were gated into a CD4 vs CD8 plot. CD4+ and CD8 + T cells were subtyped by evaluating each population using CD45RA and CD62L to differentiate naive from memory cells. To distinguish natural killer (NK) and B cells, CD3-negative cells were gated into a plot comparing CD19 (B cells) and CD16+56 (NK cells).
The guavaSoft® operating system software provides access to modules for acquisition and analysis, as well as instrument setup and maintenance. The guavaSoft® system includes templates for use with a wide range of MilliporeSigma flow cytometry kits to simplify your experiments and data collection. Additionally, the guavaSoft® package includes InCyte™, an intuitive open software package for custom analysis. Results can be exported to spreadsheets or as industry-standard FCS 2.0 or 3.0 files for further analysis. GuavaSoft® software includes 21 CFR Part 11-enabling features.

InCyte™ Software: Intuitive

Our InCyte™ software has an intuitive, easy-to-use interface that enables you to focus on data at the sample or experimental level. The software simplifies setup and analysis of plots with drag-and-drop features, while automated compensation makes it easy to perform complex, multi-color assays. The instant update feature responds in real time to change analysis conditions for viewing. The multiparameter heat mapping function allows analysis of entire plates of data in the time previously required to analyze a single sample. These features provide a simple and rapid means to attain a macroscopic view of experiment “hits” and easily compare different experiments in real time. InCyte™ software is especially useful for interpreting the results of high-throughput cell-based assays.

Small Particle Detection
The Guava® easyCyte 8HT and 12HT systems have been shown to detect particles as small as 0.2 μm, a significant improvement over typical flow cytometers. This increased resolution and sensitivity means better separation, making gating and identification of dim populations easier. These capabilities may prove particularly useful for researchers analyzing particulates, beads, bacteria or algae. Acquisition of a mixture of beads of known size demonstrates the ability of Guava® easyCyte 12HT instruments to detect and discriminate particles as small as 0.2 μm.

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