PART 2: BENCHTOP CYTOMETRY WITHOUT COMPROMISES
FOCUS ON THE SCIENCE
The CytoFLEX Flow Cytometer provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. The system can be configured for the needs of your laboratory, whether it is routine low complexity analysis, high complexity analysis, or analysis that pushes the boundaries for flow cytometry.
CELL PROLIFERATION ANALYSIS
Combining BrdU incorporation to assess actively proliferating cells and 7-AAD to measure the proportion of live cells is an effective combination for assessing key population characteristics. The CytoFLEX Flow Cytometer is ideal for these routine analyses.
Cell Cycle Analysis of proliferating human peripheral blood mononuclear cells by BrdU incorporation and 7-AAD staining.
T CELL SUBSET ANALYSIS
The complexity of heterogeneous cell population analysis continues to increase as more markers are needed to differentiate functional cell sub populations. With up to 13 channels for fluorescent detection, the CytoFLEX Flow Cytometer has the capabilities needed to meet the increased immunophenotyping demands.
Analyses of T cell subsets based on the differential expression of surface molecules related to cell function, differentiation, or activation have evolved. As a result, T cell analysis requires a multitude of markers to capture the various populations. Combining the CytoFLEX Flow Cytometer with the 13 color DuraClone tube presented here, allows for the identification of most T cell memory subpopulations discriminated by surface expression makers.
OPTIMAL RESOLUTION FOR LOW EXPRESSED ANTIGENS
Low expressed antigens and low abundance cell types are pushing the boundaries of flow cytometry. The CytoFLEX Flow Cytometer has the sensitivity and resolution capabilities to identify populations with these characteristics.
Regulatory T cells characterized by low levels of expression were easily identified.
The boundaries for flow cytometry are also being pushed by the need to measure and evaluate characteristics of smaller particles. Several fundamental capabilities of flow cytometry make this an attractive platform for extracellular vesicles, ability to detect large numbers of events, and discriminate rare events, while collecting information on phenotypic expression. The CytoFLEX Flow Cytometer has the resolution to detect particles down to 200 nm while also collecting information in the fluorescent channels.
Bead based small particles were detected using forward scatter and VSSC. Data provided by Dr. Huang Yijun, Sun Yat-Sen University, School of Medicine, Guangzhou, China.
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