PART 1: BENCHTOP CYTOMETRY WITHOUT COMPROMISES
QNORM RESOLUTION TEST RESULTS
This metric measures the number of bound antibodies that can be resolved from unstained lymphocytes with lower numbers equaling better low-end resolution.
GIGAMIX BEAD ANALYSIS
The Gigamix solution is a mixture of fluorescent Megamix-Plus SSC and Megamix-Plus FSC beads (BioCytex a Stago group company, Marseille, France) which have different sizes, 100, 160, 200, 240, 300, 500, 900 nm (red, purple, orange, blue, green, olive, and fuchsia, respectively). Beads were measured at flow rates of 10, 60 and 120 μl/min. The population of the 100 nm beads is very small and within the range of noise. Increasing the flow rate makes the 100 nm beads more visible. The visibility of all other peaks was also improved indicating that by increasing the flow rate the background noise is not also increased.
LINEARITY OF DIMLY RESPONSIVE CHANNELS
Using 8-peak beads, the third brightest peak’s median intensity was measured at every gain and plotted versus MFI.
Rainbow 8-peak Bead Data
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